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Vol. 90. Núm. 3.
Páginas 286-292 (maio - junho 2014)
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Vol. 90. Núm. 3.
Páginas 286-292 (maio - junho 2014)
ARTIGO ORIGINAL
Open Access
Oxidant-antioxidant status in Egyptian children with sickle cell anemia: a single center based study
Estado oxidante-antioxidante em crianças egípcias com anemia falciforme: estudo baseado em um único centro
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4189
Mona Kamal El-Ghamrawya,
Autor para correspondência
melghamrawi@yahoo.com

Corresponding author.
, Wagdi Maurice Hannab, Amina Abdel-Salama, Marwa M. El-Sonbatyb, Eman R. Younessc, Ahmed Adelb
a Departamento de Pediatria, New Children's Hospital, Universidade do Cairo, Cairo, Egito
b Departamento de Saúde Infantil, Centro Nacional de Pesquisa, Cairo, Egito
c Departamento de Bioquímica Médica, Centro Nacional de Pesquisa, Cairo, Egito
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Abstract
Objective

the present study was conducted to investigate the oxidant-antioxidant status in Egyptian children with sickle cell anemia.

Methods

the serum levels of total antioxidant capacity (TAO), paraoxonase (PON), vitamin E, nitrite, and malondialdehyde (MDA) were measured in 40 steady state children with homozygoussickle cell anemia (24 males and 16 females) and 20 apparently healthy age- and gender-matched controls

Results

mean serum TAO, PON, vitamin E, and nitrite levels were significantly lower in thegroup with sickle cell anemia, whereas mean serum MDA was significantly higher in these children compared to controls. No significant differences in mean levels of TAO, PON, nitrite, vitamin E, and MDA were found in sickle cell anemia patients receiving hydroxyurea when com-pared with those not receiving hydroxyurea. A significant negative correlation between serumnitrite and the occurrence of vaso-occlusive crises (VOC) was observed (r = −0.3, p = 0.04). PON level was found to be positively correlated with patients’ weight and BMI (r = −0.4, p = 0.01; r = −0.7, p < 0.001, respectively), but not with frequency of VOC. The area under the curve ofserum nitrite in predicting occurrence of VOC was 0.782, versus 0.701 for PON, and 0.650 forTAO (p = 0.006). Serum MDA was not correlated with nitrite, PON, TAO, or vitamin E levels. No significant correlations were detected between serum nitrite and hemoglobin or antioxidant enzymes.

Conclusion

children with sickle cell anemia have chronic oxidative stress that may result inincreased VOC, and decreased serum nitrite may be associated with increases in VOC frequency. A novel finding in this study is the decrease in PON level in these patients, which is an interesting subject for further research.

Keywords:
Antioxidants
Nitrite
Paraoxonase
Malondialdehyde
Sickle cell anemia
Children
Resumo
Objetivo

o presente estudo foi realizado com o objetivo de investigar o estado oxidan- te-antioxidante em crianc¸as egípcias com anemia falciforme.

Métodos

dosamos os níveis séricos da capacidade antioxidante total (CAT), paraoxonase (PON), vitamina E, nitrito e malondialdeído (MDA) em 40 crianças estáveis com anemia falciforme homozigótica (24 meninos e 16 meninas), e 20 controles pareados por idade/sexo aparente-mente saudáveis.

Resultados

os níveis séricos médios da CAT, PON, vitamina E e nitrito foram significati- vamente menores, ao passo que o nível sérico médio de MDA foi significativamente maior em crianças com anemia falciforme (AF), em comparação aos controles. Não foram encontradasdiferenças significativas nos níveis médios de CAT, PON, nitrito, vitamina E e MDA em pacientescom AF em tratamento com hidroxiureia, em comparação aos que receberam hidroxiureia. Encontramos uma correlação negativa significativa entre o nitrito sérico e a ocorrência decrises vaso-oclusivas agudas (CVO) (r = −0,3, p = 0,04). Descobrimos que o nível de PON está correlacionado positivamente com o peso e o IMC dos pacientes (r = −0,4; p = 0,01; r = −0,7; p < 0,001, respectivamente), porém não com a frequência de CVO. A área sob a curva (ASC) donitrito sérico na previsão da ocorrência de CVO foi 0,782, em comparação a 0,701 para PON e 0,650 para CAT (p = 0,006). O MDA não está correlacionado a nitrito, PON, CAT ou vitamina E. Não foram detectadas correlações significativas entre nitrito sérico e hemoglobina ou enzimas antioxidantes.

Conclusão

crianças com AF apresentam estresse oxidativo crônico que pode resultar emaumento das CVO. Em crianças com AF, a redução nos níveis de nitrito sérico pode estar associada a aumentos da frequência de CVO. Um novo achado neste estudo é a redução no nível de PON em pacientes com AF, que é um campo interessante de novas pesquisas.

Palavras-chave:
Antioxidantes
Nitrito
Paraoxonase
Malondialdeído
Anemia falciforme
Crianças
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Como citar este artigo: El-Ghamrawy MK, Hanna WM, Abdel-Salam A, El-Sonbaty MM, Youness ER, Adel A. Oxidant-antioxidant status in Egyptian children with sickle cell anemia: a single center based study. J Pediatr (Rio J).2014;90:286-92.

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