Milk from Brazilian women presents secretory IgA antibodies and neutralizes rotavirus G9P[5]

Santos, Simone M.R.; Ferreira, Thalita L.; Quintal, Virgínia S.; Carbonare, Solange B.; Tino-De-Franco, Milene

doi:10.1016/j.jpedp.2012.12.010

Article information

Abstract

Objective

To verify the presence of anti-rotavirus serotype G9P[5] SIgA and the virus neutralization capacity of milk samples from Brazilian women.

Methods

SIgA antibody levels reactive to rotavirus G9 were determined in 30 maternal milk samples by enzyme-linked immunosorbent assay (ELISA) using purified virus suspensions. The samples’ capacity to neutralize rotavirus G9P[5] was analyzed using the MA-104 cells neutralization assay.

Results

Great individual variations were observed regarding the SIgA levels and neutralization titers, but all samples showed some G9P[5] neutralizing ability. A highly significant positive correlation was observed between antibody levels and neutralization titers.

Conclusions

The high correlation between anti-rotavirus antibody levels and neutralizing capacity of the milk samples suggests a possible protective role of these antibodies against infection. These results also support the encouragement of the breast-feeding practice.

Keywords:

Human milk

Rotavirus

Secretory IgA

Rotavirus neutralization

Resumo

Objetivo

Verificar a presença de SIgA anti-rotavírus sorotipo G9P[5] e a capacidade de neutralização do vírus de amostras de leite de mulheres brasileiras.

Métodos

Foram determinados os níveis de anticorpos SIgA reativos contra rotavírus G9 em 30 amostras de leite materno por ELISA usando suspensões purificadas do vírus. A capacidade das amostras de neutralizarem o rotavírus G9P[5] foi analisada em ensaio de de Neutralização utilizando células MA-104.

Resultados

Foram observadas grandes variações individuais referentes aos níveis de SIgA e títulos de neutralização, mas todas as amostras mostraram certa capacidade de neutralizar o G9P[5]. Verificamos uma correlação positiva altamente significativa entre os níveis de anticorpos e os títulos de neutralização.

Conclusões

A alta correlação entre níveis de anticorpos anti-rotavírus e a capacidade neutralizante das amostras de leite sugere um possível papel protetor desses anticorpos contra a infecção. Esses resultados também apoiam o incentivo à prática do aleitamento materno.

Palavras-chave:

Leite humano

Rotavírus

IgA secretora

Neutralização do rotavírus

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☆

Como citar este artigo: Santos SM, Ferreira TL, Quintal VS, Carbonare SB, Tino-De-Franco M. Milk from Brazilian women presents secretory IgA antibodies and neutralizes rotavirus G9P[5]. J Pediatr (Rio J). 2013;89:510-3.

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