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January - February 2013 The impact of cystic fibrosis on the immunologic profile of pediatric patients
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Vol. 89. Issue 1.
Pages 40-47 (January - February 2013)
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Vol. 89. Issue 1.
Pages 40-47 (January - February 2013)
Original article
DOI: 10.1016/j.jpedp.2012.08.004
Open Access
The impact of cystic fibrosis on the immunologic profile of pediatric patients
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Daniela M. Bernardia,
Corresponding author
dani_miotto@yahoo.com.br

Corresponding author.
, Antonio F. Ribeirob, Tais N. Mazzolac, Maria M.S. Vilelad, Valdemiro C. Sgarbierie
a MSc in Food and Nutrition. Department of Food and Nutrition, School of Food Engineering, Universidade Estadual de Campinas (UNICAMP), Campinas, São Paulo, Brazil
b PhD in Child and Adolescent Health. Center for Investigation in Pediatrics, Department of Pediatrics, Medical School, UNICAMP, Campinas, São Paulo, Brazil
c MSc in Child and Adolescent Health. Center for Investigation in Pediatrics, Department of Pediatrics, Medical School, UNICAMP, Campinas, São Paulo, Brazil
d PhD in Biology (Immunology). Center for Investigation in Pediatrics, Department of Pediatrics, Medical School, UNICAMP, Campinas, São Paulo, Brazil
e PhD in Nutrition. Department of Food and Nutrition, School of Food Engineering, UNICAMP, Campinas, São Paulo, Brazil
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Abstract
Objective

To compare the immunologic state of 44 pediatric patients with cystic fibrosis (CF) with a control group consisting of 16 healthy individuals.

 Methods

CF patients aged 3 to 12 years with moderate to good clinical score were selected for the study. Erythrocytic glutathione, production of reactive oxygen species, cytokines (TNF-a, IFN-g, IL-8, IL-6, IL-10) in peripheral blood mononuclear cells cultures under spontaneous and BCG- or PHA-stimulated conditions, serum concentrations of TGF-b2, IgA, IgG, IgM, IgE, and salivary IgA were evaluated.

 Results

The spontaneous production of TNF-a, IL-6, and IL-10, the PHA-stimulated production of IL-6, and the serum TGF-b2, IgA, and IgG were increased in samples from CF patients. Healthy subjects had a higher production of TNF-a in response to BCG.

 Conclusion

Although CF patients appeared clinically stable, the results of their peripheral blood examinations demonstrated an impact on the immune system.

Keywords:
Cystic fibrosis
Reactive oxygen species
Glutathione
Cytokines
Immunoglobulins
Resumo
Objetivo

Comparar o estado imunológico de 44 pacientes pediátricos com fibrose cística (FC) a um grupo-controle formado por 16 indivíduos saudáveis.

 Métodos

Foram selecionados para o estudo pacientes com FC com idade entre 3 e 12 anos, apresentando um escore clínico moderado e bom. Foram avaliados a glutationa eritrocitária, a produção de espécies reativas de oxigênio, citocinas (TNF-a, IFN-g, IL-8, IL-6, IL-10) em culturas de células mononucleares do sangue periférico em condições espontâneas e estimuladas por BCG ou PHA, a concentração sérica de TGF-b2, IgA, IgG, IgM, IgE e IgA salivar.

 Resultados

A produção espontânea de TNF-a, IL-6 e IL-10, a produção de IL-6 estimula- da por PHA e TGF-b2, IgA e IgG séricas aumentaram em amostras de pacientes com FC. Indivíduos saudáveis tiveram uma produção mais elevada de TNF-a em resposta a BCG.

 Conclusão

Apesar de os pacientes com FC parecerem clinicamente estáveis, os resul- tados de seus exames de sangue periférico mostraram que houve um impacto sobre o sistema imunológico.

Palavras-chave:
Fibrose cística
Espécies reativas de oxigênio
Glutationa
Citocinas
Imunoglobulinas
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☆

Please, cite this article as: Bernardi DM, Ribeiro AF, Mazzola TN, Vilela MM, Sgarbieri VC. The impact of cystic fibrosis on the immunologic profile of pediatric patients. J Pediatr (Rio J). 2013;89:40-47.

Copyright © 2013. Sociedade Brasileira de Pediatria

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