TY - JOUR T1 - Variants in the interleukin 8 gene and the response to inhaled bronchodilators in cystic fibrosis JO - Jornal de Pediatria (English Edition) T2 - AU - Furlan,Larissa Lazzarini AU - Ribeiro,José Dirceu AU - Bertuzzo,Carmen Sílvia AU - Salomão Junior,João Batista AU - Souza,Dorotéia Rossi Silva AU - Marson,Fernando Augusto Lima SN - 00217557 M3 - 10.1016/j.jped.2017.03.005 DO - 10.1016/j.jped.2017.03.005 UR - https://jped.elsevier.es/en-variants-in-interleukin-8-gene-articulo-S0021755717306344 AB - ObjectiveInterleukin 8 protein promotes inflammatory responses, even in airways. The presence of interleukin 8 gene variants causes altered inflammatory responses and possibly varied responses to inhaled bronchodilators. Thus, this study analyzed the interleukin 8 variants (rs4073, rs2227306, and rs2227307) and their association with the response to inhaled bronchodilators in cystic fibrosis patients. MethodsAnalysis of interleukin 8 gene variants was performed by restriction fragment length polymorphism of polymerase chain reaction. The association between spirometry markers and the response to inhaled bronchodilators was evaluated by Mann–Whitney and Kruskal–Wallis tests. The analysis included all cystic fibrosis patients, and subsequently patients with two mutations in the cystic fibrosis transmembrane conductance regulator gene belonging to classes I to III. ResultsThis study included 186 cystic fibrosis patients. There was no association of the rs2227307 variant with the response to inhaled bronchodilators. The rs2227306 variant was associated with FEF50% in the dominant group and in the group with two identified mutations in the cystic fibrosis transmembrane conductance regulator gene. The rs4073 variant was associated with spirometry markers in four genetic models: co-dominant (FEF25–75% and FEF75%), dominant (FEV1, FEF50%, FEF75%, and FEF25–75%), recessive (FEF75% and FEF25–75%), and over-dominant (FEV1/FVC). ConclusionsThis study highlighted the importance of the rs4073 variant of the interleukin 8 gene, regarding response to inhaled bronchodilators, and of the assessment of mutations in the cystic fibrosis transmembrane conductance regulator gene. ER -